NCBC Roadmap apprenticeship  X-Ray Crystallography Apprenticeship

With the support of the North Carolina Biotechnology Center and the University of North Carolina at Chapel Hill, the Carolina Roadmap program is pleased to offer a 5-day apprenticeship in the Biomolecular X-ray Crystallography Core Facility (http://xtal.med.unc.edu/XRAY/index.htm).

The apprenticeship will be taught by Dr. Laurie Betts, the director of the core, and will include the following topics:

- Lectures on protein crystallography, protein crystallization, data collection and processing, synchrotron X-ray data collection, methods of structure determination, introduction to the CCP4 program suite for data analysis and structure solution, model building with COOT freeware, refinement of crystal structures, depositing structure in the protein data bank, structure validation, automation and high throughput structural genomics, PyMol program for display of protein structures.

- Demonstrations and hands-on use of software.

- Hands-on work in lab: you will solve the structure of hen egg white lysozyme.

Only 5 trainees can be accommodated. They can be graduate students, fellows, or faculty members. In choosing participants, Dr. Betts will give first preference to trainees who are not currently in crystallography labs.

The class will meet at the facility on the School of Medicine campus every Tuesday in May 2007 (1, 8, 15, 22, 29) from 9am to 5pm. You must commit to attend all 5 classes.

 

You must have a laptop that you can bring to each class. Windows and Linux are preferred, but if you are confident that you can download relevant software on your Mac, you are welcome to bring it.

 

There is no cost to attend this program.

Karen Stone, karen_stone@med.unc.edu