VS200 running ASW v3.4.1 (Draft & incomplete)

Power Up:

Check Antivibration table legs - There should be a ~3 to ~7 mm gap between top of metal ring and bottom to rubber ring at each leg.  Adjust red knob(s) 1/8th of a turn and wait ~30 seconds and then recheck gap. 


Start VS200 ASW 200 software

 

Home Screen:

Clean objective if previous use included any slides with immersion oil (currently unlikely)
Otherwise choose "Yes"
("No" can also be chosen to change trays)

 

 

 

Exchange Trays:

Note correct orientation of 6 slide tray

Load clean slides with coverslip up & slide label to the back

Insert tray gently into hotel and push tray to left (THIS IS IMPORTANT!)

 

Note: 40X Objective Correction Collar:

Use the "Clean objectives" to access the 40x objective inside the smaller box to the left

Set to 0.17 (mm) for #1.5 coverslips

 

 

Select Batch Scan:

 

 

 

 

 

Scan Project:

Select "Brightfield"

Click on first Tray in left hand column of numbers

Skip to Gallery - unless many trays in hotel need to be excluded. Then use "Tray" selection

Select Gallery

Select Brightfield | Fluorescence | Special

 

 

 

 

 

 

 

 

 

 

 

 

Select slides

left_click on 1st slide, hold shift, left_click on last slide 

&/or  control +left_mouse_click to toggle slide selection on or off 

chosen a project to apply project to selected slides

 

 

Hit "Edit scan settings" to get to overview mode

 

Overview mode:

+Overview

+Detail

+Focusing

+Naming and Saving

+Slide properties

+Scan Area Creation and Sample Detection

 

 

Check through and change if necessary:

-Overview

"Expert"

"Scan Label"

 

 

 

 

 

 

-Detail

Choose object magnification

If 40x check that correction collar is set to correct coverslip thickness (usually 0.17 mm)

Z-planes - usually "Normal" (one plane)

 

 

 

 

 

-Focusing

Focus Method: - "Prefocus"

Focus position density: "Normal"

Non-sample regions during detail scan: "Include in Scan"  off | on | Include in Focusing

 

 

 

 

 

-Naming and Saving

Mostly leave as is

Directory: - set as required. Suggest use D: drive directory

 

 

 

 

 

 

-Slide properties

 

 

Set "Slide Name" to a value.  Note can change in "Detail" mode after scanning slide labels using label information

 

 

 

 

 

-Scan Area Creation and Sample Detection (Can setup defaults now. But can mostly adjust in "Details" mode later)

Scan Area creation

Sample Detection

 

 

If settings should be applied to all slides (e.g. if change any of above from what is setup in chosen Project)

Select and highlight slide which has been setup (probably already chosen)

"Identical Settings"

 

Start Scan (acquires  overviews) & will end up in -> Detail Mode

 

 

 

Detail Mode:

 

 

 

 

 

 

 

+Detail

Can change object mag here

Can change |Z-planes" here

 

 

 

 

 

 

+Focusing

Optionally can change settings

 

 

 

 

 

 

 

+Naming and Saving

Optionally can change settings

 

 

 

 

 

 

 

+Slide Properties - can change Slide name and other slide properties (new in version 3.4.1)

Optionally can change settings

Can change slide name for each slide based on information in label scans

 

 

 

 

 

 

For each slide:

Check/set Scan areas

 

 

 

 

 

 

 

 

Do Focus maps for each slide

Remove focus square which are over nothing (machine cannot focus on nothing. 

Move/remove focus square which are not fill to ~50% with tissue

Could change "Include in Scan" to on or "Include in Focusing" to on

 

 

 

 

 

Start Scan

 

scanning in progress

Can check scans by double clicking on slide when scan completed or even during scan

Click on "Return to scanning"

Can open "Open Log File" to check on slide scan completion progress

 

 

 

 

Finish Mode:

(again) Can check scans by double clicking on slide when scan completed or even during scan

Hit Home on lower right when done.

 

 

 

 

 

 

 

 

Fluorescence notes are yet to be created for ASW 3.4.1

Note instructions in green are for fluorescence and can be skipped for transmitted light imaging