Table 1. Properties of Alexa Fluor Dyes

  Alexa Fluor dye Absorption
max. (nm)		 Emission
max (nm)		 Emission color* Extinction
coefficient**
1 Alexa Fluor 350 346 442 Blue 19,000
2 Alexa Fluor 405 401 421 Blue 34,000
3 Alexa Fluor 430 433 541 Green/Yellow 16,000
4 Alexa Fluor 488 496 519 Green 71,000
7 Alexa Fluor 532 532 553 Yellow 81,000
8 Alexa Fluor 546 556 573 Orange 104,000
9 Alexa Fluor 555 555 565 Orange 150,000
10 Alexa Fluor 568 578 603 Orange/Red 91,000
11 Alexa Fluor 594 590 617 Red 73,000
12 Alexa Fluor 610 612 628 Red 138,000
13 Alexa Fluor 633 632 647 Far Red 239,000
14 Alexa Fluor 635 633 647 Far Red 140,000
15 Alexa Fluor 647 650 665 Near-IR*** 239,000
16 Alexa Fluor 660 663 690 Near-IR*** 132,000
17 Alexa Fluor 680 679 702 Near-IR*** 184,000
18 Alexa Fluor 700 702 723 Near-IR*** 192,000
19 Alexa Fluor 750 749 775 Near-IR*** 240,000
20 Alexa Fluor 790 784 814 Near-IR*** 270,000

 

https://www.thermofisher.com/us/en/home/brands/molecular-probes/key-molecular-probes-products/alexa-fluor/alexa-fluor-dyes-across-the-spectrum/jcr:content/MainParsys/textimage_8719/image.img.320.medium.png/1559738966592.png

 

 

Absorbance and emission spectra of non-sulfonated cyanine dyes

 

 

 

 


Opal Fluorophores

Wavelength

Excitation

Emission

Opal 520

494 nm

525 nm

Opal 540

523 nm

536 nm

Opal 570

550 nm

570 nm

Opal 620

588 nm

616 nm

Opal 650

627 nm

650 nm

Opal 690

676 nm

694 nm

Spectral DAPI

358 nm

461 nm

 

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Fluorescent Protein Database https://www.fpbase.org/spectra/ (hit space bar and enter a fluorescent protein acronym, e.g. eGFP)