Alexa Fluor dye | Absorption max. (nm) |
Emission max (nm) |
Emission color* | Extinction coefficient** |
|
---|---|---|---|---|---|
1 | Alexa Fluor 350 | 346 | 442 | Blue | 19,000 |
2 | Alexa Fluor 405 | 401 | 421 | Blue | 34,000 |
3 | Alexa Fluor 430 | 433 | 541 | Green/Yellow | 16,000 |
4 | Alexa Fluor 488 | 496 | 519 | Green | 71,000 |
7 | Alexa Fluor 532 | 532 | 553 | Yellow | 81,000 |
8 | Alexa Fluor 546 | 556 | 573 | Orange | 104,000 |
9 | Alexa Fluor 555 | 555 | 565 | Orange | 150,000 |
10 | Alexa Fluor 568 | 578 | 603 | Orange/Red | 91,000 |
11 | Alexa Fluor 594 | 590 | 617 | Red | 73,000 |
12 | Alexa Fluor 610 | 612 | 628 | Red | 138,000 |
13 | Alexa Fluor 633 | 632 | 647 | Far Red | 239,000 |
14 | Alexa Fluor 635 | 633 | 647 | Far Red | 140,000 |
15 | Alexa Fluor 647 | 650 | 665 | Near-IR*** | 239,000 |
16 | Alexa Fluor 660 | 663 | 690 | Near-IR*** | 132,000 |
17 | Alexa Fluor 680 | 679 | 702 | Near-IR*** | 184,000 |
18 | Alexa Fluor 700 | 702 | 723 | Near-IR*** | 192,000 |
19 | Alexa Fluor 750 | 749 | 775 | Near-IR*** | 240,000 |
20 | Alexa Fluor 790 | 784 | 814 | Near-IR*** | 270,000 |
Opal Fluorophores |
Wavelength |
|
Excitation |
Emission |
|
Opal 520 |
494 nm |
525 nm |
Opal 540 |
523 nm |
536 nm |
Opal 570 |
550 nm |
570 nm |
Opal 620 |
588 nm |
616 nm |
Opal 650 |
627 nm |
650 nm |
Opal 690 |
676 nm |
694 nm |
Spectral DAPI |
358 nm |
461 nm |
Fluorescent Protein Database https://www.fpbase.org/spectra/ (hit space bar and enter a fluorescent protein acronym, e.g. eGFP)