Michael Hooker Microscopy Facility Tutorial Workshop

Light Microscopy for Biomedical Research
Workshop September 22 to 26, 2008

The Michael Hooker Microscopy Facility, UNC is offering a one week workshop on the use of light microscopy in biomedical research. The workshop is intended to provide an introduction to the basic concepts of light microscopy and its application to biological research with lectures, hands on sessions and industry involvement.

For more information contact: Michael Chua lmbr@mhmf.org 843-3268

Join the wait list for the workshop: microscopy.unc.edu/registration

Light Microscopy for Biomedical Research - September 22-26, 2008
Monday - Sept 22 010 Bondurant Hall	Tuesday - Sept 23 010 Bondurant Hall	Wednesday - Sept 24 010 Bondurant Hall	Thursday - Sept 25 010 Bondurant Hall	Friday - Sept 26 010 Bondurant Hall
8:45 Registration 9:00 Intro. - History of Light Microscopy, Modes of imaging, Parts of a Light Microscope - M.C.	9:00 Talk - Fluorescence, labels, filters, dichroics, live cell imaging, cross talk.	9:00 Talk - Confocal Microscopy I - principle, scanning - M.C.	9:00 Talk - Advanced confocal, spinning disk, colocalization - M.C. 10:00 Multiphoton Confocal - Chris Fecko	9:00 Invited Talks - 3-D Image Deconvolution - Nicholas Beavers
10:30 Objectives, Kohler illumination, Image formation - M.C.	10:30 Invited Talk - Immuno labeling, cell labeling - Neal Kramarcy 11:00 Live cell imaging - M. Chua	10:00 Confocal II continued - PMT, multichannel, 3D, projections- M.C. 11:00 Total Internal Reflection Fluorescence - Linda Brown	11:00 Fluorescence and FRAP - Jeffrey Harris	10:00 Segmentation and tracking Vazha Glonty 11:00 Invited Talk - Visualization and Representation - David Feng
13:00 Hands on 417 MacNider Hall - parts of a microscope, care, digital acquisition - alignment	13:00 Invited Talk 4052 Bondurant - 1. CCD cameras, noise, color/monochrome - Hilary Hicks 2. Digital images, sampling, resolution, Nyquist	13:00 Hands on 417 MacNider - Confocal I - sensitivity, pinhole, filters, averaging, zoom pixel size z-series, time lapse, simultaneous/sequential, Colocalization	13:00 Hands on 417 MacNider - Confocal II - multi-channel, FRAP, Rendering, 3D, projections 3-D Deconvolution	13:00 Hands on 417 MacNider - movies, scaling compression, resizing, conversion, format for Power Point, Imaging tools - Visualization Tools - Feng, Marschburn, Vazha Glonty , Chua,
16:00 Invited Talk 010 Bondurant - DIC/Phase Contrast - Ted Salmon	14:45 Hands on 417 MacNider - Widefield fluorescence	16:00 Invited Talk 010 Bondurant - Fluorescent Biosensors for Living Cells - Klaus Hahn	16:00 Invited Talks 010 Bondurant - Light sources - M. Chua, White light laser - Juergen von der Heiden	15:30 Finish
Finish 17:30	Finish 5:30 PM Dinner 6:30 PM	Finish 17:30	Finish 17:30
Schedule is subject to change

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