Light Microscopy for Biomedical Research Workshop - September 21 to 25, 2009 - University of North Carolina - Chapel Hill

A one week workshop on the use of light microscopy in biomedical research is being offered by the Michael Hooker Microscopy Facility, UNC.  The workshop is intended to provide an introduction to the basic concepts of light microscopy and its application to biological research with lectures, hands on sessions and industry involvement.

 

 
 

To attend register at: http://microscopy.unc.edu/registration
 
Monday - Sept 21   9:00 AM to 5:30 PM   Light Microscopy, modes of imaging, parts of a light microscope, objectives, Kohler illumination, image formation, Hands on - parts of a microscope, care, digital acquisition - alignment, Invited Talk -  DIC/Phase Contrast
 
Tuesday - Sept 22   9:00 AM to 5:30 PM   Fluorescence, Jablonski diagram, contrast, filters, cross talk, immuno labeling, cell labeling, hands on  CCD cameras,  noise, color/monochrome, digital images, sampling, resolution, Nyquist, Dinner 6:00 PM
 
Wednesday - Sept 23   9:00 AM to 5:30 PM   Confocal  Microscopy, principles, scanning, PMTs, AOTF, multichannel, 3D, projections, hands on confocal I -  sensitivity, pinhole, filters, averaging, zoom pixel size z-series, time lapse, simultaneous/sequential, Colocalization, Invited Talk -  Fluorescent Biosensors for Living Cells
 
Thursday - Sept 24   9:00 AM to 5:30 PM   Advanced confocal, 2 photon, spinning disk, colocalization, digital images, Fluorescence and FRAP, Hands on confocal II - multi-channel, FRAP, rendering, 3D, projections, 3-D  deconvolution, Light sources
 
Friday - Sept 25   9:00 AM to 4:00 PM   3-D Image deconvolution, 3-D Visualization, Tracking, 3-D rendering, Visualization and Representation, Hands on - movies, scaling compression, resizing, conversion, formatting for Power Point, imaging tools
 
Note: Daily topics and schedule are subject to change
 
Figures credits: left - "Brainbow" - Alain Burette; right  - Isolated epithelial cell - Rob Wonsetler & Larry Ostrowski
 
Tuition and Tuesday dinner: $350 - please note new rates. (People from labs of full Cancer Center members $150; Members of CF, GT & Alcohol Centers & the Jin lab. $50.  These reduced rates reflect the contribution these groups have made to the operation of the MHMF)