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Light Microscopy for Biomedical Research -
September 21-25, 2009 - Room 305 Mary Ellen Jones Bldg |
| Monday - Sept 21 |
Tuesday - Sept 22 |
Wednesday - Sept 23 |
Thursday - Sept 24 |
Friday - Sept 25 |
8:45 Check in, coffee, doughnuts
9:00 Intro. - History of Light Microscopy, Modes of imaging, Parts of a
Light Microscope |
9:00 Talk - Fluorescence, labels, filters,
dichroics, live cell imaging, cross talk. |
9:00 Talk - Confocal Microscopy I -
principle, scanning. |
9:00 Talk - Advanced confocal, spinning disk, colocalization
10:15 Multiphoton Confocal - Robert Peterson
10:45 Zeiss 700 - Johnafel Crowe
11:00 Nikon Swept scan - Jim Samso |
9:00 Image Processing segmentation
introduction (Metamorph) - Jim Sims |
| 10:30 Objectives, Kohler
illumination, Image formation 11:30 Cameras - CCD 1 - Hilary
Hicks |
10:30 Immuno labeling, cell labeling - Neal
Kramarcy
11:00 Live cell imaging - FRET 11:30 Cameras - CCD 2 - Hilary
Hicks |
10:00 Confocal II continued - PMT,
multichannel, 3D, projections. 11:15 Confocal imaging optimization -
Eva Johannes |
11:15 Multichannel, Fluorescence, Live Cell
Imaging - Mike Meade |
10:00 Colocalization - Digital images, sampling, resolution |
13:00 Hands on
parts of a microscope, care, digital acquisition -
alignment |
13:00 Hands on
Widefield fluorescence |
13:00 Hands on
- Confocal I - sensitivity,
pinhole, filters, averaging, zoom pixel size z-series, time
lapse, FRAP, simultaneous/sequential, Colocalization |
13:00 Hands on
- Confocal II - multi-channel, FRAP, Rendering, 3D,
projections
3-D Deconvolution |
13:00 Invited Talk - Visualization and Representation - Dr. Russell
Taylor 14:15 Hands
on
Imaris, Image Surfer, partical tracking, static background removal,
movies, scaling compression, resizing, conversion, format for Power
Point |
16:00 Invited Talk
DIC/Phase Contrast - Dr. Ted Salmon |
16:00 Invited Talk
- Fluorescent Biosensors for Living Cells - Dr. Klaus Hahn |
16:00 Invited Talks
- Studying Cell Dynamics with Fluorescent Techniques - Dr. Ken Jacobson |
16:00 Light sources for microscopy. |
16:00 Decompression - Serious drinks, snacks
and chat! |
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18:00 Dinner - Carolina Brewery |
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Location - room 305 Mary Ellen Jones
Building, UNC, Chapel Hill Topics & schedule are subject to
change |
The
Michael Hooker Microscopy Facility, UNC is offering a one week workshop on the
use of light microscopy in biomedical research. The workshop is intended
to provide an introduction to the basic concepts of light microscopy and its
application to biological research with lectures, hands on sessions and industry
involvement.
