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Workshop: Light Microscopy for
Biomedical Research -
September
20 to 24, 2010 - University of North Carolina - Chapel Hill |
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A one week workshop on
the use of light microscopy in biomedical research is being offered by the Michael Hooker Microscopy Facility,
UNC. The workshop is
intended to provide an introduction to the basic concepts of light microscopy
and its application to biological research with lectures, hands on sessions and industry
involvement.
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To attend
please register
at:
http://microscopy.unc.edu/registration |
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| Monday - Sept 20 9:00 AM to 5:30 PM Light Microscopy, modes of imaging, parts of a light
microscope, objectives, Kohler illumination, image formation, Hands on - parts of a microscope, care, digital
acquisition - alignment, Invited Talk - DIC/Phase Contrast |
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| Tuesday - Sept 21 9:00 AM to 5:30 PM Fluorescence, Jablonski diagram, contrast, filters, cross
talk, immuno labeling, cell labeling, hands on CCD cameras, noise, color/monochrome,
digital images, sampling, resolution, Nyquist, Dinner 6:00 PM |
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| Wednesday - Sept 22 9:00 AM to 5:30 PM Confocal Microscopy, principles, scanning, PMTs,
AOTF, multichannel, 3D, projections, hands on confocal I - sensitivity, pinhole,
filters, averaging, zoom pixel size z-series, time lapse,
simultaneous/sequential, Colocalization, Invited Talk - Fluorescent Biosensors for Living Cells |
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| Thursday - Sept 23 9:00 AM to 5:30 PM Advanced confocal, 2 photon, spinning disk, colocalization,
digital images, Fluorescence and FRAP, Hands on confocal II - multi-channel, FRAP,
rendering, 3D,
projections, 3-D deconvolution, Light sources |
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| Friday - Sept 24 9:00 AM to 4:00 PM 3-D Image
deconvolution, 3-D Visualization, Tracking, 3-D
rendering, Visualization and Representation, Hands on - movies, scaling compression, resizing,
conversion, formatting for Power Point, imaging tools |
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Note: Daily topics and schedule are subject to change |
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Figures credits: left - "Brainbow" - Alain Burette; right -
Isolated epithelial cell - Rob Wonsetler &
Larry Ostrowski |
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Tuition and Tuesday dinner: $600 |