Light Microscopy for Biomedical Research
Workshop 2010 is postponed

The Michael Hooker Microscopy Facility, UNC - Chapel Hill is offering a one week workshop on the use of light microscopy in biomedical research.  The workshop is intended to provide an introduction to the basic concepts of light microscopy and its application to biological research with lectures, hands on sessions and industry involvement.

Due to circumstances beyond our control this works has been postponed.  We hope to reschedule this event in the future and apologize for the inconvenience.

For more information contact:
Michael Chua   microscopy@unc.edu  843-3268
Neal_Kramarcy@med.unc.edu  966-7051

Join the notification list for the postponed 2010 or early 2011 workshop: microscopy.unc.edu/registration
Note due to family reasons the workshop has to be postponed until early 2011

 

Light Microscopy for Biomedical Research - September 20-24, 2010 postponed until early 2011

Monday - Sept 20 Tuesday - Sept 21 Wednesday - Sept 23 Thursday - Sept 23 Friday - Sept 24
8:45 Registration
9:00 Intro. - History of Light Microscopy, Modes of imaging, Parts of a Light Microscope
9:00 Talk - Fluorescence, labels, filters, dichroics, live cell imaging, cross talk. 9:00 Talk - Confocal  Microscopy I - principle, scanning. 9:00 Talk - Advanced confocal,  spinning disk, colocalization

10:00 Multiphoton Confocal

9:00 Invited Talks - 3-D Image Deconvolution
10:30  Objectives, Kohler illumination, Image formation 10:30 Invited Talk - Immuno labeling, cell labeling

11:00 Live cell imaging

10:00 Confocal II continued - PMT, multichannel, 3D, projections.

11:00 Total Internal Reflection Fluorescence

11:00 Fluorescence and FRAP 10:00 Segmentation and tracking

11:00 Invited Talk - Visualization and Representation

13:00 Hands on
parts of a microscope, care, digital acquisition - alignment
13:00 Invited Talk
1. CCD cameras,  noise, color/monochrome
2. Digital images, sampling, resolution, Nyquist
13:00 Hands on
- Confocal I - sensitivity, pinhole, filters, averaging, zoom pixel size z-series, time lapse, simultaneous/sequential, Colocalization
13:00 Hands on
- Confocal II - multi-channel, FRAP, Rendering, 3D, projections
3-D  Deconvolution
13:00 Hands on
- movies, scaling compression, resizing, conversion, format for Power Point, Imaging tools - Visualization Tools
16:00 Invited Talk
DIC/Phase Contrast
14:45 Hands on
Widefield fluorescence
16:00 Invited Talk
- Fluorescent Biosensors for Living Cells

16:00 Invited Talks
- Light sources

 
         

Schedule and specific topics are subject to change