Light Microscopy for
Biomedical Research
Workshop 2010 is
postponed
The Michael Hooker Microscopy Facility, UNC - Chapel Hill is offering a one week workshop on the use of light microscopy in biomedical research. The workshop is intended to provide an introduction to the basic concepts of light microscopy and its application to biological research with lectures, hands on sessions and industry involvement. Due to circumstances beyond our control this works has been postponed. We hope to reschedule this event in the future and apologize for the inconvenience. For more information
contact:
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Light Microscopy for Biomedical Research - September 20-24, 2010 postponed until early 2011 |
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Monday - Sept 20 | Tuesday - Sept 21 | Wednesday - Sept 23 | Thursday - Sept 23 | Friday - Sept 24 |
8:45 Registration 9:00 Intro. - History of Light Microscopy, Modes of imaging, Parts of a Light Microscope |
9:00 Talk - Fluorescence, labels, filters, dichroics, live cell imaging, cross talk. | 9:00 Talk - Confocal Microscopy I - principle, scanning. | 9:00 Talk - Advanced confocal, spinning disk, colocalization 10:00 Multiphoton Confocal |
9:00 Invited Talks - 3-D Image Deconvolution |
10:30 Objectives, Kohler illumination, Image formation | 10:30 Invited Talk - Immuno labeling, cell labeling 11:00 Live cell imaging |
10:00 Confocal II continued - PMT,
multichannel, 3D, projections. 11:00 Total Internal Reflection Fluorescence |
11:00 Fluorescence and FRAP | 10:00 Segmentation and tracking 11:00 Invited Talk - Visualization and Representation |
13:00 Hands on parts of a microscope, care, digital acquisition - alignment |
13:00 Invited Talk 1. CCD cameras, noise, color/monochrome 2. Digital images, sampling, resolution, Nyquist |
13:00 Hands on - Confocal I - sensitivity, pinhole, filters, averaging, zoom pixel size z-series, time lapse, simultaneous/sequential, Colocalization |
13:00 Hands on - Confocal II - multi-channel, FRAP, Rendering, 3D, projections 3-D Deconvolution |
13:00 Hands
on - movies, scaling compression, resizing, conversion, format for Power Point, Imaging tools - Visualization Tools |
16:00 Invited Talk DIC/Phase Contrast |
14:45 Hands on Widefield fluorescence |
16:00 Invited Talk - Fluorescent Biosensors for Living Cells |
16:00 Invited Talks |
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Schedule and specific topics are subject to change |