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Volocity - 3D & Time Lapse Viewing, Analysis, Quantitation, Tracking and Deconvolution
Note: This information page is available on line at: http://microscopy.unc.edu/Volocity

This page is for historical purposes and contains useful information

What is Volocity:

Volocity v6.3 (Perkin Elmer/formerly Improvision/new ) is a 3D and time lapse (4D) imaging program for:

• Volume visualization - volume rendered, ray tracing, orthogonal views, maximum projection, stereo, VR
• 3-D/4-D Image processing
• Analysis
• Object measurement
• Tracking (including 3-D)
• Co-localization analysis in 2-D or 3-D
• Deconvolution - blind or measured point spread function for confocal or widefield microscopes
• Stack realignment

It can use data from diverse sources such as confocal microscopes & widefield microscopes.  Volocity can easily import z-series &/or time lapse image sequences with from the Zeiss 510/7x0 or Leica SP2/SP5/SP8 or Olympus FV1000/1200 or Biorad or Perkin Elmer confocal programs with meta data, C-Imaging data, as well as .tif and other image format image series.

Where can it be Used:

Volocity is installed on the image processing workstations in the Michael Hooker Microscopy Fellowship:

• \\Schott (this computer is temporarily on vacation) high performance work stations - (Windows 7 - 64 bit, 8 GByte RAM, 1 GB OpenGL video)
• On your computer!  You will need to have a dedicated account to use the Volocity license server.  Volocity runs on Macs, Windows 32 & Windows 64 bit.  Obtain the appropriate Volocity software from the Perkin Elmer Volocity web site, install it, and connect to the license server.  For adequate performance your computer should have sufficient RAM (>= 4 GBytes and a high performance OpenGL video card.  Volocity installation software version 6.3 can be also downloaded from the \\minsky.med.unc.edu\Users2\1-softwares/Volocity file server (MHmicroscopy account required). 

Who Can Use Volocity:

Users will need to arrange to have obtain this software (see just above) before being able to use the license server.  Most users will use the user name and password issued by Cell Biology & Physiology (Robert Currin).  Much analysis only requires one license and not two or all three.  The Volocity LE viewer and image database organizer is free, but an account and connection to the license server is required. 

License Availability:

Available individually on a first come first served basis to users with Volocity accounts.  Licenses availability is shown on the license server web page: http://152.19.37.206:5900

• Available Modules (please choose selected modules carefully in order to have the desired features available):
  • Volocity (Volocity LE) - "Light Edition" Image importing/exporting, cropping, resizing, viewing and image library organization only - Instant access to Volocity (LE)
  • Volocity Visualization (Vis) - 3D rendering
  • Volocity Quantitation (Quant) - Measurement, tracking & co-localization
  • Volocity Restoration (Restor) - Deconvolution  (blind or measured)
• Combined Modules
  • Volocity Vis & Quant - 3D rendering and measurement, tracking & co-localization
  • Volocity Vis & Restor - 3D & Deconvolution
  • Volocity Vis & Quant & Restor - Deconvolution

• Volocity uses an on line license server to assign a user license instance to run the program.  See section 1. below.
• Users who have not used Volocity via the license server should contact facility personnel to obtain an account.
• Volocity 6.3 latest available manual:
  • Version 6 manual
    • http://microscopy.unc.edu/Resources/Volocity/VolocityUserGuide60.pdf
  • From Perkin Elmer/Improvision/Volocity web site :
    • http://www.improvision.com/support/guides/volocity.php
• Application examples from Improvision

1.  Using Volocity