Michael Hooker Microscopy Facility

Michael Hooker Microscopy Facility (MHMF.ORG)

Reference

Microscopy & Imaging Books	Location
Confocal and Two-Photon Microscopy: Foundations, Applications and Advances, Alberto Diaspro (Editor) 2001 Imaging Neurons - A Laboratory Manual, R. Yuste, F. Lanni & A. Konnerth, 2000	M. Chua
Optical Imaging Techniques in Cell Biology, Guy Cox	Health Sciences Library + MHMF
Video Microscopy The Fundamentals, First Edition, Shinya Inoue	M. Chua
Video Microscopy The Fundamentals, Second Edition, Shinya Inoue and Kenneth R. Spring 1997	M. Chua
Electronic Light Microscopy, D. Shotton (ed.), Wiley-Liss, 1993	M. Chua
Handbook of Biological Confocal Microscopy, 2nd ed, J. B. Pawley (ed.), Plenum Press, 1995	MHMF
Handbook of Biological Confocal Microscopy, 3rd ed J. B. Pawley (ed.), Plenum Press, 2006	MHMF
The Image Processing Handbook. J. C. Russ, CRC Press, 1st, 2nd, 3rd & 4th editions	UNC libraries
The Image Processing Handbook. 4th Edition, J. C. Russ, CRC Press, 2002	MHMF
Confocal Microscopy for Biologists BIOCON, publishers	Duke Perkins library
Unbiased Stereology, CV Howard and MG Reed	M. Chua

Basic Methods in Microscopy: Protocols And Concepts from Cells, a Laboratory Manual, Spector & Goldman	Missing?
Principles of Fluorescence Spectroscopy [electronic resource] / edited by Joseph R. Lakowicz, 2006	On line
http://webcat.lib.unc.edu/search?/tPrinciples+of+Fluorescence+Spectroscopy/tprinciples+of+fluorescence+spectroscopy/1%2C1%2C4%2CB/frameset&FF=tprinciples+of+fluorescence+spectroscopy&4%2C%2C4
Molecular Imaging, FRET Microscopy and Spectroscopy, Periasamy and Day, 2005	Zoology Library
Methods in Cellular Imaging, Periasamy, 2001	Zoology Library

Some Publications from the facility

Articles of Interest

GFP

A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells, (2002) George H. Patterson and Jennifer Lippincott-Schwartz, Science 2002, 1873-1877. [Supporting Online Material]

http://www.ncbi.nlm.nih.gov/entrez/utils/fref.fcgi?http://www.biochemj.org/bj/356/0345/bj3560345.htm

Quantum Dots

Quantum dots, Kouwenhoven
In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles, (2002) Benoit Dubertret, Paris Skourides, David J. Norris, Vincent Noireaux, Ali H. Brivanlou, and Albert Libchaber, Science, 298, 1759-1762

Immuno

Anti GFP supplier

Bryan R. Hewlett, "Penetration Rates of Formaldehyde", Microscopy Today,
Vol 10 (6), 30, Nov/Dec, 2002.

Clearing

Bucher, D. et al., Journal of Neuroscience. Methods 100 (2000), 135-143.

Interference Techniques

G. A. Dunn, Transmitted-light interference microscopy: a technique born before its time, Proceedings of the Royal Microscopical Society, 33, 189-196, 1998.

Live Cell Imaging

Lepe-Zuniga, J.L., Zigler, J.S. & Gery, I. Toxicity of light-exposed Hepes media. J Immunol Methods 103:145-150 (1987).

Knight MM, Roberts SR, Lee DA, Bader DL. Live cell imaging using confocal microscopy induces intracellular calcium transients and cell death. Am J Physiol Cell Physiol. 2003 Apr;284(4):C1083-9.Stereology

Stereology

CV Howard and MG Reed: "Unbiased Stereology"

PR Mouton: "Principles and practices of unbiased stereology"

Manuals	Location
Adobe Illustrator 10 manual, 2002, on loan to Lisa	6007 TB
Adobe Premiere 6 manual, 2001, on loan to Lisa	6007 TB
Adobe Photoshop 7 manual, 2002	6129 TB


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Last Updated: 2014-07-24

Copyright 2001-2015 Dr. M. Chua, School of Medicine, University of North Carolina, Chapel Hill, NC 27599

Questions/comments/problems: Michael Chua