Michael Hooker Microscopy Facility

microscopy.unc.edu

At the University of North Carolina

The Michael Hooker Microscopy Facility is a research microscopy facility in the Thurston Bowles building at the University of North Carolina, Chapel Hill.

The facility provides standard and advanced digital light microscopy and image processing resources to users from the UNC Chapel Hill campus on a fee for use basis.  Instrumentation and instruction are provided to enable users to acquire, process and analyze images from samples they have prepared, or we can acquire data with you.

Light Microscopy for Biomedical Research - a workshop at UNC Chapel Hill - September 22 to 26 (Mon to Fri)  -  9:00 AM to 5:30 PM    Registration

Information for new and current users - Users are required to complete the user information form and training with facility personnel for each microscope resource they wish use, or be assisted with scanning. Please contact Michael or Neal in order to schedule training or assisted scanning.  All users with wet or live samples must read this note.

• Mission Statement
• Facility Policies
• Charges
• New user/updated information form
• Information about MHmicroscopy Logon accounts
• After hours access to Thurston Bowles 6129, 6123 & 6033a
• All users with wet or live samples must read the note at this link

• Neal Kramarcy will be assisting at the MHMF
• Image Processing Workstation - \\Zernike now runs Volocity
• MetaMorph 7.1 is available on \\Snell & v4.5 on \\Brewster
• Fluorescence filters for far-red emitting dyes (e.g. Cy5, Alexa 647, Draq-5) on the Inverted Leica fluorescence microscope.  See Michael or Neal

• New proximity cards (see picture below) are required to enter the 6th floor doors and external doors of the Thurston Bowles building after hours.  Sch. of Med. has updated most users' cards. Please test your card before relying on it.  Contact us if you need assistance with card access.

• Aug 28: Zeiss 510 confocal - 633 nm (red) laser is not working.  The other lasers can still be used.  A replacement power supply has been ordered.
• Aug 22: Microscope stand on the Leica SP2 Confocal has been reset.  Focus and limit setting is working properly again.

• Thurston Bowles building room 6129, 6033a, 6033 & 6123
• Users phone number: 966-7051 room 6129, 6033a, 6033, 6123

• Inverted Leica fluorescence microscope with digital cameras and DIC
• Upright Nikon fluorescence microscope and dissecting scope with color CCD camera
• Inverted Nikon Microscope, TE2000 multifunction time lapse with dual cameras
• Fluorescence Dissecting Stereo Microscope /Macroscope

• Laser Scanning Confocal Microscope, Zeiss 510 meta
• Laser Scanning Confocal Microscope, Leica SP2 aobs
• Spinning Disk Confocal, Perkin Elmer
• Laser Scanning Confocal Microscope, LeicaTCS-4D

• Laser Micro-Dissection and capture, Leica AS-LMD
• Atomic Force Microscope
• Microscope stage heaters
• Color solid dye printer: Xerox Phaser 8550
• Laser jet double sided printer: HP1320
• High resolution color scanner: Epson Perfection 4180 Photo
• Humidified  Incubator
• Refrigerator (4 C) for sample and reagent storage

• Computer resources - File transfer - Network Connections - Accounts/Passwords
• Image Processing Workstations: Brewster - Snell - Young - Zernike - Gould
• Image Processing Software - Viewers - C-Imaging - Metamorph - Volocity - Image Surfer - Command prompt
• How To's - basic image handling: colorize, overlay images, etc.

Copyright 2001-08   M. Chua, Office of Research, School of Medicine, University of North Carolina, Chapel Hill, NC 27599

Last updated  09/03/08