Michael Hooker Microscopy Facility (MHMF.ORG)

Hardware Resources

• FLUORESCENCE MICROSCOPES (Wide field)
  • Inverted Leica fluorescence microscope with digital cameras and DIC
  • Upright Nikon fluorescence microscope and a dissecting microscope with a high resolution color CCD camera
  • Inverted Nikon TE2000 fluorescence, time lapse, multiple fluorescence, DIC, Phase Contrast, ratio imaging, Fura-2, Prim, CFP/YFP
  • Leica Fluorescence Stereo Micro/Macrsope
• CONFOCAL MICROSCOPES - Comparison of confocal features - another comparison
  • Spinning Disk Confocal, Perkin Elmer, Ultraview LCS
  • Laser Scanning Confocal Microscope Leica SP2 UV aobs
  • Laser Scanning Confocal Microscope Zeiss 510 meta
  • Laser Scanning Confocal Microscope LeicaTCS-4D
• MISCELLANEOUS
  • Image analysis and quantification workstations - Brewster - Snell - Young
    • Volocity 4 - Metamorph 4.6
    • Zeiss 510 - LeicaLCS 2.6 - C-Imaging
    • Adobe Creative Suite - Photoshop - Illustrator - Bridge - ImageReady -
    • MS Office 2003 - Word - Excel - Power Point - FrontPage
    • QuickTime 7
    • VidEdit
    • VHS video digitization
    • Flatbed scanner with transparency
  • Laser Micro-Dissection and capture
  • Atomic Force Microscope
  • Color solid dye printer: Xerox Phaser 860
  • Laser jet double sided printer: HP1320
  • Incubator 37 C, 5% CO2 and 95% controlled humidity
  • Refrigerator (4 C) for sample or reagent storage
  • Digital Oscilloscope
  • Laser Power Meter (Coherent )
  • Soldering iron
  • Multimeter

Software Resources

• Volocity v3.7 or 4.0  3D rendering and classification license on  \\Snell
• Zeiss LSM 3.2 - full display & analysis software on \\Snell
• Zeiss Image Browser 3.2 - available on CD or download
• LeicaTCS off line with complete analysis  - \\Snell  \\Brewster
• LeicaTCS light  - available on CD or download
• PhotoShop 7.0 or PhotoShop CS or CS2 on \\Fresnel, \\Hooke, \\Young, \\Brewster
• Premiere 6.0 on \\Young
• C-Imaging analysis - \\Brewster,  \\Young,  \\Snell  and also on acquisition stations: \\Fresnel, \\Hooke, \\Morpheus, \\Euler, \\Shiva, \\Wheatstone, \\Nipkow
• Arcobat 6 or 7
• Winzip 11.0  -  10 computer license
• System Commander
• Partition Magic
• Ghost 9
• ThumbsPlus 7 site license - \\Snell,  \\Fresnel,  \\Hooke,  \\Young  \\Brewster

How Tos

Go to How To's

Planned Resources

• 3D Deconvolution - got module for C-Imaging
• Whole embryo imaging

Planned Resources currently shelved

• FCS
• TIRF
• Fluorescent Macroscope

		Copyright 2001-08  Dr. M. Chua, Office of Research, University of North Carolina, Chapel Hill, NC 27599
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